pe-cy7 anti-mouse cd16/32 antibody  (Thermo Fisher)

Journal: American Journal of Translational Research

Article Title: Immune changes and their relationship with prognosis in patients with varicella-zoster virus encephalitis/meningitis

doi:

Figure Lengend Snippet: Immune function of patients in three groups

Article Snippet: Data collection General demographic information, gender, age, clinical characteristics (mainly including duration of disease, the site of zoster, treatment, etc.), comorbidity and history of past illness of enrolled patients were collected. . Main testing reagents and instruments BD FACSCalibur flow cytometry (US BD Company); Detection of lymphocyte subsets: monoclonal antibodies CD3FITC, CD4 PE-Cy7, CD8 APC-Cy7, CD16+/CD56 PE, CD19 APC and hemolysin (products of BD Company in the United States); Low temperature high-speed centrifuge (Heraeus, Germany); 7600-020 Automatic Biochemical Analyzer (Hitachi, Japan). .

Techniques:

Journal: American Journal of Translational Research

Article Title: Immune changes and their relationship with prognosis in patients with varicella-zoster virus encephalitis/meningitis

doi:

Figure Lengend Snippet: Immunity related variables in VZV encephalitis/meningitis patients with different prognosis

Article Snippet: Data collection General demographic information, gender, age, clinical characteristics (mainly including duration of disease, the site of zoster, treatment, etc.), comorbidity and history of past illness of enrolled patients were collected. . Main testing reagents and instruments BD FACSCalibur flow cytometry (US BD Company); Detection of lymphocyte subsets: monoclonal antibodies CD3FITC, CD4 PE-Cy7, CD8 APC-Cy7, CD16+/CD56 PE, CD19 APC and hemolysin (products of BD Company in the United States); Low temperature high-speed centrifuge (Heraeus, Germany); 7600-020 Automatic Biochemical Analyzer (Hitachi, Japan). .

Techniques:

Journal: BioMed Research International

Article Title: Differences in the Expression of KIR, ILT Inhibitory Receptors, and VEGF Production in the Induced Decidual NK Cell Cultures of Fertile and RPL Women

doi: 10.1155/2021/6673427

Figure Lengend Snippet: Mouse anti-human antibodies with conjugated fluorochromes were used in the experiment. Phenotyping was performed in 4 color schemes in three tubes. Each tube included CD56 and CD16 to gate the population of interest: in tube 1, the induction of NK cells to idNK cells and killer immunoglobulin-like receptors were assessed, and in tube 2, ILT expression was checked.

Article Snippet: The collected cells in the amount of 1 × 10 5 obtained by centrifugation of cell cultures were resuspended in 100 μ l of 0.1% NaN 3 PBS (Sigma-Aldrich), and antibodies against surface markers for CD56 conjugated with Pe-Cy7 and CD16 with FITC were added (BD, USA).

Techniques: Expressing